HRP-conjugated Rabbit anti-Human IgG1 (Fc) mAb

Artikelnummer: ABB-AS092
Artikelname: HRP-conjugated Rabbit anti-Human IgG1 (Fc) mAb
Artikelnummer: ABB-AS092
Hersteller Artikelnummer: AS092
Alternativnummer: ABB-AS092-1000UL, ABB-AS092-100UL, ABB-AS092-5X100UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Immunogen: This information is considered to be commercially sensitive.
Konjugation: HRP
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Reinheit: Affinity purification
Target-Kategorie: IgG1
Antibody Type: Secondary Antibody
Application Verdünnung: WB,1:2000 - 1:30000|ELISA, 1:5000-1:10000
Anwendungsbeschreibung: Cross-Reactivity: Human
Western blot analysis of vlysates from Human plasma using HRP-conjugated Rabbit anti-Human IgG1 (Fc) mAb (AS092) at 1:4000-1:32000 dilution.
Lysates/proteins: 25 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30 s.