HRP-conjugated Rabbit anti-Human IgG4 mAb

Artikelnummer: ABB-AS102
Artikelname: HRP-conjugated Rabbit anti-Human IgG4 mAb
Artikelnummer: ABB-AS102
Hersteller Artikelnummer: AS102
Alternativnummer: ABB-AS102-5X100UL, ABB-AS102-100UL, ABB-AS102-1000UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Spezies Reaktivität: Human
Immunogen: This information is considered to be commercially sensitive.
Konjugation: HRP
Konzentration: ELISA
UniProt: P01861
Reinheit: Affinity purification
Target-Kategorie: Human IgG4/Immunoglobulin heavy constant gamma 4
Antibody Type: Secondary Antibody
Application Verdünnung: ELISA, 1:5000-1:10000
Anwendungsbeschreibung: Cross-Reactivity: Human
Dose response curve of HRP conjugated Rabbit Anti-Human IgG4 mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Human IgG4 mAb in a 2 fold serial dilution from 1 µg/mL to 7.8*10 -3 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit Rabbit Anti-Human IgG4 has highly specific recognition of Human IgG4 while no or minimal cross reactivity to Human IgG1,Human IgG2,Human IgG3,Human IgM,Human IgE,Human IgD.