HRP-conjugated Rabbit anti-Human IgE mAb

Artikelnummer: ABB-AS104
Artikelname: HRP-conjugated Rabbit anti-Human IgE mAb
Artikelnummer: ABB-AS104
Hersteller Artikelnummer: AS104
Alternativnummer: ABB-AS104-100UL, ABB-AS104-1000UL, ABB-AS104-5X100UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Spezies Reaktivität: Human
Immunogen: This information is considered to be commercially sensitive.
Konjugation: HRP
Konzentration: ELISA
UniProt: P01854
Reinheit: Affinity purification
Target-Kategorie: Immunoglobulin heavy constant epsilo
Antibody Type: Secondary Antibody
Application Verdünnung: ELISA, 1:5000-1:10000
Anwendungsbeschreibung: Cross-Reactivity: Human
Dose response curve of HRP conjugated Rabbit Anti-Human IgE (Fc) mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Human IgE (Fc) mAb in a 2 fold serial dilution from 2 µg/mL to 6.1*10 -5 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit Anti-Human IgE (Fc) mAb has highly specific recognition of Human IgE while no or minimal cross reactivity to Human IgG1,Human IgG2,Human IgG3,Human IgG4,Human IgM,Mouse IgG,Rat IgG.