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The 7-fluorescence Human Transitional/Immature B cells Panel has been designed for identify and phenotype B cells in Human blood or tissue samples. ABflo multi-fluorescence panel RK40021 covers the core CD markers of CD45, CD19, CD10, CD38, CD21 and IgD. Our plots showed clear subgroups of CD10+CD38+IgD+ transitional B cells, CD10-CD38-IgD+ unswitched B cells, CD10-CD38-IgD- memory B cells, CD10-CD38highIgD- plasmablasts, CD10-CD38-CD27-IgD+ naive, CD10-CD38-CD27+IgD+ marginal zone B cells and AM/TLM/IM/RM Memory B cells (cite figures).
Konzentration:
FC
Application Verdünnung:
FC,5 µl per 10 6 cells in 100 µl volume
Anwendungsbeschreibung:
Cross-Reactivity: Human
Multicolor flow cytometry panel to identify Human B cell subsets. Human PBMC were surface stained with ABflo 450 Rabbit anti-Human CD45 mAb (A25195,5 µL/Test), PerCP/Cyanine5.5 Rabbit anti-Human/Monkey CD19 mAb (A26823,5 µL/Test), FITC Rabbit anti-Human CD10 mAb (A26865,5 µL/Test), ABflo 610 Rabbit anti-Human IgD mAb (A26481,5 µL/Test), ABflo 700 Rabbit anti-Human CD38 mAb (A27140,5 µL/Test), PE Rabbit anti-Human CR2/CD21 mAb (A25702,5 µL/Test), ABflo 647 Rabbit anti-Human CD27 mAb (A22064,5 µL/Test). All antibodies are validated for Flow cytometry. B cells are defined as CD45+CD19+. A major B cell subset discrimination is based on CD10 and, therefore, B cells were divided into CD10- and CD10+ subpopulations. CD10+ B cells can be further refined into transitional B cells (CD38+IgD+ ), CD10- B cells comprise IgD+ unswitched B cells (CD38-IgD+), memory B cells (CD38-IgD-) and plasmablasts (PB, CD38highIgD-). The unswitched IgD+ fraction can be further divided in naive (CD27-IgD+) and marginal zone (MZ) B cells (CD27+IgD+), Memory B cells can be further divided in activated memory (AM), tissue-like memory (TLM), intermediate memory (IM) and resting memory(RM) by expression patterns of CD21 and CD27.
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