B- and T-lymphocyte attenuator (BTLA, CD272) is a 35 kDa type I transmembrane glycoprotein in the CD28 family of T cell costimulatory molecules. BTLA is a inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2 . BTLA may interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14.
95 % as determined by Tris-Bis PAGE, 95 % as determined by HPLC.
Formulierung:
Lyophilized from a 0.22 µm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization. Contact us for customized product form or formulation.
Target-Kategorie:
BTLA
Application Verdünnung:
Lyophilized from a 0.22 µm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization. Contact us for customized product form or formulation.
Anwendungsbeschreibung:
Cross-Reactivity: Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage,it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA,5% HSA,10% FBS or 5% Trehalose),and aliquot the reconstituted protein solution to minimize free-thaw cycles., ResearchArea: Bio-Markers & CD Antigens
Biotinylated Recombinant Human BTLA/CD272 Protein was determined by Tris-Bis PAGE under reducing conditions.
The purity of Biotinylated Human BTLA is greater than 95% as determined by SEC-HPLC
Immobilized Anti-BTLA Antibody, hFc Tag at 0.5µg/ml (100µl/well) on the plate. Dose response curve for Biotinylated Human BTLA, His Tag with the EC<,sub>,50<,/sub>, of 8.0ng/ml determined by ELISA.
Immobilized Human HVEM, hFc Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human BTLA, His Tag with the EC<,sub>,50<,/sub>, of 1.4µg/ml determined by ELISA.
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