GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.
95 % as determined by Tris-Bis PAGE, 95 % as determined by HPLC.
Target-Kategorie:
GFRAL/GFR alpha-like
Anwendungsbeschreibung:
Cross-Reactivity: Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage,it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA,5% HSA,10% FBS or 5% Trehalose),and aliquot the reconstituted protein solution to minimize free-thaw cycles., ResearchArea: Other Recombinant Protein
Recombinant Human GFR alpha-like/GFRAL Protein was determined by Tris-Bis PAGE under reducing conditions.
The purity of Human GFRAL is greater than 95% as determined by SEC-HPLC.
Immobilized Human GDF15, His Tag at 0.5µg/ml (100µl/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC<,sub>,50<,/sub>, of 15ng/ml determined by ELISA.
Human GFRAL, hFc Tag captured on CM5 Chip via Protein A can bind Human GDF15, His Tag with an affinity constant of 0.014 nM as determined in SPR assay (Biacore T200).
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