Recombinant Human Ubiquitin (K29 only) protein, E. coli
Artikelnummer:
ABB-RP10072LQ
Hersteller Artikelnummer:
RP10072LQ
Alternativnummer:
ABB-RP10072LQ-50UG,ABB-RP10072LQ-25UG
Hersteller:
ABclonal
Wirt:
E. coli
Kategorie:
Proteine/Peptide
Spezies Reaktivität:
Human
Ubiquitin (Ub) is a 76 amino acid protein widely expressed in the cytoplasmic and nucleus of cells. Ub is posttranslationally conjugated to proteins by the E1, E2, E3 protein ubiquitination cascade. Ub can be conjugated on proteins as monoUb or polyUb chains. Protein ubiquitination plays both proteolytic and nonproteolytic functions. Usually, polyubiquitinated proteins are targeted to the 26S proteasome for proteolysis. Typical concentration to support in vitro ubiquitination is 50-100 µM. In Ub(K29 only), all lysine residues but lysine 29 were substituted with arginines, thereby it only supports the formation of lysine 29-linked polyubiquitin chains.
Konzentration:
Please contact us for more information.
Reinheit:
95% as determined by reducing SDS-PAGE.
Formulierung:
20 mM Tris, 150 mM NaCl, 2 mM betaME, 10% Glycerol
Target-Kategorie:
Ubiquitin (K29 only)
Application Verdünnung:
20 mM Tris, 150 mM NaCl, 2 mM betaME, 10% Glycerol
Anwendungsbeschreibung:
ResearchArea: Ubiquitination related reagent
Recombinant Human Ubiquitin (K29 only) protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
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