Monoclonal Antibody to SARS-CoV-2 Nucleocapsid (Clone: ABM3H4.1F7), Clone: [ABM3H4.1F7], Mouse

Produktübersicht

• Artikelname: Monoclonal Antibody to SARS-CoV-2 Nucleocapsid (Clone: ABM3H4.1F7), Clone: [ABM3H4.1F7], Mouse
• Artikelnummer: ABI-10-10034
• Hersteller Artikelnummer: 10-10034
• Alternativnummer: ABI-10-10034-100UG
• Hersteller: Abeomics
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Immunogen: Full length recombinant SARS-CoV-2 nucleocapsid Protein was used as the immunogen for this antibody.
• Alternative Synonym: sars-cov-2

Produktbeschreibung

The structural nucleocapsid (N) protein of nCoV/SARS-CoV-2/COVID-19 is a predicted 46 kDa phosphoprotein having 419 amino acid residues. A short Serine rich stretch and a recognized nuclear localization signal are the unique features of the nucleocapsid protein of nCoV/SARS-CoV-2/COVID-19, which seems to have a little homology with the proteins of other members of this large corona virus family. These features also suggest the involvement of nucleocapsid protein of nCoV/SARS-CoV-2/COVID-19 in different stages of viral lifecycle. The protein has multifaceted activities including packing of the nCoV/SARS-CoV-2/COVID-19 viral genome into a helical ribonucleocapsid (RNP) and playing an important role in viral self-assembly causing nCoV/SARS-CoV-2/COVID-19. The nucleocapsid protein of nCoV/SARS-CoV-2/COVID-19 also forms dimer in the cell by self-association with the help of interactive C terminal domain.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [ABM3H4.1F7]
• NCBI: 43740575
• UniProt: P0DTC9
• Reinheit: Protein G Chromatography
• Formulierung: Purified
• Target-Kategorie: Nucleocapsid
• Antibody Type: Monoclonal Antibody

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilutions: WB: 0.1-1 µg/ml, ELISA: 1 µg/ml. However, this need to be optimized based on the research applications.

Bilder

Figure-1: An indirect ELISA is carried out by coating nucleocapsid protein in serial dilution from 20 ng to 0.312 ng and using 100 ng of purified monoclonal antibodies 3H4.1F7. Peroxidase conjugated Goat-Anti mouse antibody was used at 1:5000 dilution.

Figure-3: Anti-Nucleocapsid monoclonal Antibodies (3H4.1F7) was screened against different proteins to evaluate the specificity of the antibodies.

Figure-2: Western Blot analysis: The purified antibodies 3H4.1F7 was tested on Nucleocapsid Recombinant protein at different concentrations, 0.1 (lane 1), 0.5 (lane 2), and 1.0 µg/ml (lane 3), (4) RBD protein, (5) unrelated protein 1 ,(6) unrelated protein 2, (7) unrelated protein 3, to detect the specific binding. 25 ng of proteins was loaded per lane.

Figure-4: Western Blot analysis of SARS-CoV-2 Nucleocapsid Antibody: Anti- SARS-CoV-2 Nucleocapsid Antibody (Clone: ABM3H4.1F7)) was used at 4 µg/ml on (1) SARS-CoV-2 virus infected Vero Cell lysates and (2) Mock infected lysates.