Monoclonal Antibody to CD73 (Clone: ABM40E2), Clone: [ABM40E2], Mouse

Produktübersicht

• Artikelname: Monoclonal Antibody to CD73 (Clone: ABM40E2), Clone: [ABM40E2], Mouse
• Artikelnummer: ABI-10-4106
• Hersteller Artikelnummer: 10-4106
• Alternativnummer: ABI-10-4106-100UG
• Hersteller: Abeomics
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: FACS, IHC, WB
• Spezies Reaktivität: Human
• Immunogen: A partial length recombinant CD73 protein (amino acids 145-346) was used as the immunogen for this antibody.
• Alternative Synonym: NT5E||NT5||NTE

Produktbeschreibung

CD73, also known as ecto-5-nucleotidase, is a glycosyl-phosphatidylinositol linked plasma membrane glycoprotein that is expressed on multiple cell types and in different tissues. It is a key enzyme in the regulation of purinergic signaling and inflammatory reactions. It hydrolyzes extracellular AMP into adenosine, which dampens immune cell activation, and reduces leukocyte trafficking CD73 potently suppresses antitumor T cell responses through its ability to generate adenosine. It has also emerged as an important regulator of tissue homeostasis and pathophysiologic responses related to immunity, inflammation, pain, ischemia, tissue fibrosis and cancer.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [ABM40E2]
• NCBI: 4907
• UniProt: P21589
• Reinheit: Protein G Chromatography
• Formulierung: Purified
• Target-Kategorie: CD73
• Antibody Type: Monoclonal Antibody

Anwendungsbeschreibung

• Application Verdünnung: Western blot analysis: 2-4 µg/ml, Immunohistochemical analysis: 5 µg/ml, FACS: 0.2-0.5 µg/10 6 cells

Bilder

Fig-2 : Immunohistochemical analysis of CD73in endomatrium carcinoma using CD73 antibody (Clone: ABM40E2) at 5 µg/ml.

Fig-6: Cell Surface flow analysis of hCD73 in PBMC (Lymphocytes) using 0.2µg/10 6 cells of CD73 clone ( ABM40E2). Green represents isotype control, red represents anti-hCD73 antibody. Goat anti-mouse PE conjugated secondary antibody (ABEOMICS) was used.

Fig-3: Immunohistochemical analysis of CD73in human Liver using CD73 antibody (Clone: ABM40E2) at 5 µg/ml.

Fig-4: Immunohistochemical analysis of CD73in human Spleen using CD73 antibody (Clone: ABM40E2) at 5 µg/ml.

Fig-1: Immunohistochemical analysis of CD73 in renal cell carcinoma using CD73 antibody (Clone: ABM40E2) at 5 µg/ml.

Fig-5: Western blot analysis of CD73. Anti- CD73 antibody (Clone: ABM40E2) was tested at 2 µg/ml on human liver lysate.