Monoclonal Antibody to CLEC4E (Clone: ABM42C6), Clone: [ABM42C6], Mouse
Artikelnummer:
ABI-10-4120-25
Hersteller Artikelnummer:
10-4120-25
Alternativnummer:
ABI-10-4120-25-25UG
Hersteller:
Abeomics
Wirt:
Mouse
Kategorie:
Antikörper
Applikation:
FACS, WB
Spezies Reaktivität:
Human
Immunogen:
A full length human CLEC4E protein was used as the immunogen for this antibody.
Alternative Synonym:
CLEC4E||CLECSF9||MINCLE||UNQ218/PRO244
CLEC4E (C-type lectin domain family 4, member E), also called as Mincle, is a type II transmembrane C-type lectin receptor that specifically binds to the cord factor TDM (trehalose-6,6-dimycolate), a unique glycolipid of mycobacterial cell-surface components, and activates immune cells to confer adjuvant activity. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signaling, glycoprotein turnover, and roles in inflammation and immune response. CLEC4E is mainly expressed in myeloid cells, such as macrophages and neutrophils and possesses a single carbohydrate-recognition domain (CRD) in the extracellular region. It is associated with FcRgamma chain, an immunoreceptor tyrosine-based activation motif (ITAM)-containing adaptor, and acts as an activating receptor that senses damaged cells. CLEC4E is also a downstream target of CCAAT/enhancer binding protein (C/EBP), beta (CEBPbeta) and may play a role in inflammation.
Western blot analysis: 2-4 µg/ml, Flowcytometric analysis: 0.5-1 µg/10 6 cells
Figure-1: Western blot analysis of CLEC4E. Anti-CLEC4E antibody (Clone: ABM42C6) was tested at 2 µg/ml on THP-1 lysate.
Figure-2: cell surface flowcytometric analysis of Anti-CLEC4E in A431 cells using 0.5 µg/10 6 cells of Anti-CLEC4E (clone: ABM42C6) . Green represent isotype control and red represent Anti-CLEC4E antibody (10-4120 ABEOMICS). Goat anti-mouse PE conjugate was used as the secondary antibody.
Figure-3: cell surface flowcytometric analysis of Anti-CLEC4E in HeLa cells using 0.5 µg/10 6 cells of Anti-CLEC4E (clone: ABM42C6) . Green represent isotype control and red represent Anti-CLEC4E antibody (10-4120 ABEOMICS). Goat anti-mouse PE conjugate was used as the secondary antibody.
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