Western blot analysis: 2-4 µg/ml, Facs analysis- 0.5-1 µg/10 6 Cells
Figure:1- Western blot analysis of Galectin-1. Anti-Galectin-1 antibody (Clone: ABM55A5) was tested at 0.01 µg/ml on (1) Recombinant protein and 2 µg/ml on (2) HCT-116, (3) Hela, (4) 3T3, (5) 293, (6) K562, (7) THP1 and (8) PC3 lysates.
Figure:2- Intracellular flow analysis of Galectin-1 in U87 cell line using 0.5 µg/10 6 cells of Galectin-1 antibody (Clone: ABM55A5). Green represents isotype control, red represents anti-Galectin-1 antibody (10-7619). Goat anti-mouse PE conjugate was used as secondary antibody.
Figure:3- Intracellular flow analysis of Galectin-1 in A431 cell line using 0.5 µg/10 6 cells of Galectin-1 antibody (Clone: ABM55A5). Green represents isotype control, red represents anti-Galectin-1 antibody (10-7619). Goat anti-mouse PE conjugate was used as secondary antibody
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