Source: Escherichia Coli.Sterile Filtered colorless solution.MDH2 catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. MDH2 is localized to the mitochondria and takes part in the malate-aspartate shuttle that functions in the metabolic coordination between cytosol and mitochondria. MDH2 is highy expressed in the adrenal system, small intestine, heart and pancreas.MDH2 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 335 amino acids (25-338a.a.) and having a molecular mass of 35.4kDa. MDH2 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Reinheit:
Greater than 95.0% as determined by SDS-PAGE.
Application Verdünnung:
Specific activity is > 800 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxaloacetate and beta-NADH to Lmalate and beta-NAD per minute at pH8.0 at 37C.
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