C-Terminal 2/3rds of Xenopus cdc2 expressed in E.coli
Alternative Synonym:
Cdc2, CDC28A, CDK1, CDKN1, CELL CYCLE CONTROLLER CDC2, Cell division control protein 2 homolog, Cell division cycle 2 G1 to S and G2 to M, Cell division protein kinase 1, Cyclin-dependent kinase 1, p34 Protein Kinase, P34CDC2
Recognizes a 34kDa protein (cdk1), identified as p34cdc2 (a catalytic subunit of Maturation Promoting Factor). Its epitope maps near the C-terminus of the protein and its core is thoµght to be LGTPNNEV (aa220-227 in murine cdc2). It shows no cross reaction with cdk2 p32. It supports the kinase activity. p34cdc2 plays a crucial role during cell division and is most active during mitosis. It is a serine/threonine kinase, which is activated by cyclin, by dephosphorylation of tyrosine residues. p34cdc2 is inactivated by a tyrosine kinase. This MAb reportedly inhibits the activation of p34cdc2 kinase by cyclins.
Inhibits activation of p34cdc2 kinase by cyclins,,Immunofluorescence (1-2ug/ml), Immunoprecipitation, Kinase Assay, Western Blot, ,Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating t
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