It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[rBRD.3]
Antibody Type:
Monoclonal Antibody
Application Verdünnung:
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) [For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37oC
Fig. 1: SDS-PAGE Analysis Puriifed BrdU Recombinant Mouse Monoclonal Antibody (rBRD.3). Confirmation of Purity and Integrity of Antibody.
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