Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an alpha chain (36kDa) and a beta chain (27kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six alpha and ten beta chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response.
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM
Fig. 1: Formalin-fixed, paraffin-embedded human Tonsil stained with HLA-Pan Mouse Recombinant Monoclonal Antibody (rHLA-Pan/3475).
Fig. 2: SDS-PAGE Analysis Purified HLA-Pan Mouse Recombinant Monoclonal Antibody (rHLA-Pan/3475). Confirmation of Purity and Integrity of Antibody.
Fig. 3: Flow Cytometric Analysis of Human Raji cells using HLA-Pan Mouse Recombinant Monoclonal Antibody (rHLA-Pan/3475)followed by Goat anti-Mouse IgG-CF488 (Blue), Isotype control (Red).
Fig. 4: Immunofluorescence Analysis of Human Raji cells with HLA-Pan Mouse Recombinant Monoclonal Antibody (rHLA-Pan/3475) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).
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