It recognizes polypeptides of 58kDa, 56kDa, and 45kDa, identified as cytokeratin 5, 6, and 18 respectively. It shows no reaction with keratin 1, 8, or 19. This MAb shows a broad pattern of reactivity with epithelial tissues, from simple glandular epithelia to stratified squamous epithelia. Epithelial cells are labeled whether they are ectodermal, mesodermal, or endodermal in origin. This MAb is useful in detecting cells of epithelial origin in mixed tumors, in effusions, in bone marrow samples, or in tissue culture cells.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[LP34]
Antibody Type:
Monoclonal Antibody
Application Verdünnung:
Immunohistochemistry (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes),
Fig. 1: Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin, 5/6/18 Mouse Monoclonal Antibody (LP34).
Fig. 2: SDS-PAGE Analysis Purified Cytokeratin, 5/6/18 Mouse Monoclonal Antibody (LP34). Confirmation of Purity and Integrity of Antibody.
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