Anti-Cytokeratin, Multi (Epithelial Marker) Monoclonal Antibody(Clone: KRT/1877R), Clone: [KRT/1877R], Rabbit
Artikelnummer:
ABI-36-3807-20
Hersteller Artikelnummer:
36-3807-20
Alternativnummer:
ABI-36-3807-20-20UG
Hersteller:
Abeomics
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
FACS, IF, IHC, WB
Spezies Reaktivität:
Human, Mouse, Rat
Immunogen:
Keratin-enriched preparation from cultured human epithelial cells
Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59kDa (CK4), 58kDa (CK5), 56kDa (CK6), 52kDa (CK8), 56.5kDa (CK10), 53kDa (CK13) and 45kDa (CK18). This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA,
Fig. 1: Formalin-fixed, paraffin-embedded human Skin stained with Multi-Cytokeratin Recombinant Rabbit Monoclonal Antibody (KRT/1877R).
Fig. 2: SDS-PAGE Analysis Purified Multi-Cytokeratin Rabbit Monoclonal Antibody (KRT/1877R). Confirmation of Integrity and Purity of Antibody.
Fig. 3: Immunofluorescence Analysis of MeOH-fixed Human MCF-7 cells labeling CK with Multi-Cytokeratin Recombinant Rabbit Monoclonal Antibody (KRT/1877R)followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).
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