The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al.(1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.
Western blot : 0.25-0.5µg/mlµg/ml, Immunohistochemistry(Paraffin-embedded Section) : 0.5-1µg/ml
Figure 1. Western blot analysis of beta-Actin using anti-beta-Actin antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50µg of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: monkey kidney tissue lysates, Lane 4: monkey liver tissue lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysat
Figure 2: Anti-beta-Actin antibody(monoclonal) 39-1062. Western blotting: Lane 1: Rat Liver Tissue Lysate, Lane 2: Rat Spleen Tissue Lysate, Lane 3: Rat Brain Tissue Lysate, Lane 4: Rat Kidney Tissue Lysate, Lane 5: HELA Cell Lysate, Lane 6: SMMC Cell.
