Human Bcl -2 (B cell Lymphoma 2) Pre-Coated ELISA Kit

Produktübersicht

• Artikelname: Human Bcl -2 (B cell Lymphoma 2) Pre-Coated ELISA Kit
• Artikelnummer: ABI-90-2186
• Hersteller Artikelnummer: 90-2186
• Alternativnummer: ABI-90-2186-1X96TESTS
• Hersteller: Abeomics
• Kategorie: Sonstiges
• Applikation: ELISA
• Spezies Reaktivität: Human

Produktbeschreibung

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human Bcl-2 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human Bcl-2 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Bcl-2 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450 nm in a microplate reader. Concentration of Human Bcl-2 can be calculated using the standard curve.

Anwendungsbeschreibung

• Application Verdünnung: This immunoassay kit allows for the in vitro quantitative determination of Human Bcl-2 concentrations in serum, plasma and other biological fluids. Detection Range: 0.156 - 10 ng/ml Sensitivity: <0.094 ng/ml