This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IL-13 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-13 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-13 amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader, and then the concentration of IL-13 can be calculated.
Application Verdünnung:
This immunoassay kit allows for the in vitro quantitative determination of Mouse IL-13 concentrations in serum, plasma and other biological fluids. Detection Range: 31.25 - 2000 pg/ml Sensitivity: < 18.75 pg/ml
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