This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Mouse PDL-1 antibody was pre-coated into 96-well plates. Biotin conjugated anti-Mouse PDL-1 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse PDL-1 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450 nm in a microplate reader. Concentration of Mouse PDL-1 can be calculated using the standard curve.
Application Verdünnung:
This immunoassay kit allows for the in vitro quantitative determination of Mouse PDL-1 concentrations in serum, plasma and other biological fluids. Detection Range: 0.156 - 10 ng/ml Sensitivity: <0.094 ng/ml
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