Y-79 (human retinoblastoma) whole cell lysate (non-denatured), Human
Artikelnummer:
ABN-L042V2
Hersteller Artikelnummer:
L042V2
Alternativnummer:
ABN-L042V2-200
Hersteller:
Abnova
Wirt:
Human
Kategorie:
Zellen/Zellkultur
Applikation:
IP, WB
Spezies Reaktivität:
Human
Whole cell lysate (non-denatured).
Puffer:
In modified RIPA Lysis Buffer.
Quelle:
Retina
Application Verdünnung:
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Anwendungsbeschreibung:
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Mi
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