Y-79 (human retinoblastoma) nuclear extract lysate (denatured), Human
Artikelnummer:
ABN-L042V3
Hersteller Artikelnummer:
L042V3
Alternativnummer:
ABN-L042V3-50
Hersteller:
Abnova
Wirt:
Human
Kategorie:
Zellen/Zellkultur
Applikation:
WB
Spezies Reaktivität:
Human
Nuclear extract cell lysate (denatured).
Puffer:
In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue).
Quelle:
Retina
Application Verdünnung:
The lysate is ready to load on SDS-PAGE for Western blotting.
Anwendungsbeschreibung:
Nuclear extract was prepared by using a modified protocol of Dignam et al. Cells were Harvested and homogenized in Buffer A, and then centrifugated at 25,000 g for 20 minutes to remove cytoplasm and pellet the nuclei. The pellet was re-suspended in Buffe
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