Y-79 (human retinoblastoma) nuclear extract lysate (non-denatured), Human
Artikelnummer:
ABN-L042V4
Hersteller Artikelnummer:
L042V4
Alternativnummer:
ABN-L042V4-50
Hersteller:
Abnova
Wirt:
Human
Kategorie:
Zellen/Zellkultur
Applikation:
IP, WB
Spezies Reaktivität:
Human
Nuclear extract cell lysate (non-denatured).
Puffer:
In Buffer D.
Quelle:
Retina
Application Verdünnung:
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Anwendungsbeschreibung:
Nuclear extract was prepared by using a modified protocol of Dignam et al. Cells were Harvested and homogenized in Buffer A, and then centrifugated at 25,000 g for 20 minutes to remove cytoplasm and pellet the nuclei. The pellet was re-suspended in Buffe
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