The Fas is also known as FAS receptor (FasR), apoptosis antigen 1 (APO1 or APT), cluster of differentiation 95 (CD95) or tumor necrosis factor receptor superfamily member 6 (TNFRSF6). is a death receptor on the surface of cells that leads to programmed cell death (apoptosis). It is one of two apoptosis pathways, the other being the mitochondrial pathway. FasR is located on chromosome 10 in humans and 19 in mice. Similar sequences related by evolution (orthologs) are found in most mammals. Fas forms the deathinducing signaling complex (DISC) upon ligand binding. Membraneanchored Fas ligand trimer on the surface of an adjacent cell causes trimerization of Fas receptor. This event is also mimicked by binding of an agonistic Fas antibody, though some evidence suggests that the apoptotic signal induced by the antibody is unreliable in the study of Fas signaling. To this end, several clever ways of trimerizing the antibody for in vitro research have been employed.Upon ensuing death domain (DD) aggregation, the receptor complex is internalized via the cellular endosomal machinery. This allows the adaptor molecule FADD to bind the death domain of Fas through its own death domain. Recently, Fas has also been shown to promote tumor growth, since during tumor progression, it is frequently downregulated or cells are rendered apoptosis resistant. Cancer cells in general, regardless of their Fas apoptosis sensitivity, depend on constitutive activity of Fas. This is stimulated by cancerproduced Fas ligand for optimal growth. - Antikörper
Tag:
Mouse IgG1 | Mouse kappa
Puffer:
PBS, pH7.4, 0.03% Proclin300, 0.2% BSA
Formulierung:
Liquid
Target-Kategorie:
CD95
Anwendungsbeschreibung:
Flow Cytometry (Evaluation of the expression of CD95 on Human cells).
FABm035-01-50tests
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
