Biotinylated Human FGF basic Protein, Avitag(TM),His Tag (HPLC verified), E. coli

Artikelnummer: ABS-FGC-H81E3-25UG
Artikelname: Biotinylated Human FGF basic Protein, Avitag(TM),His Tag (HPLC verified), E. coli
Artikelnummer: ABS-FGC-H81E3-25UG
Hersteller Artikelnummer: FGC-H81E3-25ug
Alternativnummer: ABS-FGC-H81E3-25UG
Hersteller: AcroBiosystems
Wirt: E. coli
Kategorie: Proteine/Peptide
Spezies Reaktivität: Human
Konjugation: Biotin
FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 3560% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF basic has been isolated from a number of sources, including neural tissue, pituitary, adrenal cortex, corpus luteum, and placenta. This factor contains four cysteine residues, but reduced FGF basic retains full biological activity, indicating that disulfide bonds are not required for this activity. bFGF is a critical component of human embryonic stem cell culture medium, the growth factor is necessary for the cells to remain in an undifferentiated state, although the mechanisms by which it does this are poorly defined. It has been demonstrated to induce gremlin expression which in turn is known to inhibit the induction of differentiation by bone morphogenetic proteins. It is necessary in mousefeeder cell dependent culture systems, as well as in feeder and serumfree culture systems. - Proteine/Peptide
Molekulargewicht: 20.1 kDa
Tag: N-Avi & N-10*His
NCBI: 09038
Puffer: 50 mM Tris, 150 mM NaCl, pH7.5
Reinheit: 90%
Formulierung: Powder
Target-Kategorie: FGF basic
Immobilized Human Glypican 3, Fc Tag, low endotoxin (Cat. No. GP3-H5258) at 5 µg/mL (100 µL/well) can bind Biotinylated Human FGF basic, Avitag,His Tag (Cat. No. FGC-H81E3) with a linear range of 0.6-10 ng/mL (QC tested).
Immobilized Human FGFR2 (IIIb), His Tag (Cat. No. FGB-H5223) at 5 µg/mL (100 µL/well) can bind Biotinylated Human FGF basic, Avitag,His Tag (Cat. No. FGC-H81E3) with a linear range of 0.01-0.313 µg/mL (Routinely tested).
Biotinylated Human FGF basic, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
FGC-H81E3-25ug