Biotinylated Human PD-1 / PDCD1 Protein, Avitag(TM),His Tag (recommended for biopanning) (MALS verified)

Produktübersicht

• Artikelname: Biotinylated Human PD-1 / PDCD1 Protein, Avitag(TM),His Tag (recommended for biopanning) (MALS verified)
• Artikelnummer: ABS-PD1-H82E4-200UG
• Hersteller Artikelnummer: PD1-H82E4-200ug
• Alternativnummer: ABS-PD1-H82E4-200UG
• Hersteller: AcroBiosystems
• Wirt: Human
• Kategorie: Proteine/Peptide
• Spezies Reaktivität: Human
• Konjugation: Biotin

Produktbeschreibung

Programmed cell death protein 1 (PD1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD1 has two ligands, PDL1 and PDL2, which are members of the B7 family. PDL1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFNgamma. PDL2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the Tcell proliferation and production of related cytokines including IL1, IL4, IL10 and IFNgamma by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCRmediating signal by dephosphorylating key signal transducer. In vitro, treatment of antiCD3 stimulated T cells with PDL1Ig results in reduced T cell proliferation and IFNgamma secretion. Monoclonal antibodies targeting PD1 that boost the immune system are being developed for the treatment of cancer. - Proteine/Peptide

Produkteigenschaften

• Molekulargewicht: 18.6 kDa
• Tag: C-Avi & C-6*His
• NCBI: 15116
• Puffer: PBS, pH7.4
• Reinheit: 95%
• Formulierung: Powder
• Target-Kategorie: PD-1

Anwendungsbeschreibung

• Anwendungsbeschreibung: PD1-H82E4 works best for experiments that test the binding between PD-1 and candidate antibodies, such as biopanning and other relevant assays. This product is NOT suitable for testing PD1-PDL1 binding by ELISA. For this type of application, we strongly recommend you to choose PD1-H82F1 as an alternative.

Bilder

PD1-H82E4-200ug

Immobilized Nivolumab at 2 µg/mL (100 µL/well) can bind Biotinylated Human PD-1 Protein, Avitag,His Tag (Cat. No. PD1-H82E4) with a linear range of 0.8-13 ng/mL (QC tested).

Immobilized Human VEGF165, His Tag (Cat. No. VE5-H5248) at 2 µg/mL, add increasing concentrations of Ivonescimab and then add Biotinylated Human PD-1 Protein, Avitag,His Tag (Cat. No. PD1-H82E4) at 2 µg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) with sensitivity of 2.17 ng/mL (Routinely tested).

Biotinylated Human PD-1 Protein, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.