Biotinylated SARS-CoV-2 S protein (HV69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H) trimer, His,Avitag(TM) (MALS verified), Human

Produktübersicht

• Artikelname: Biotinylated SARS-CoV-2 S protein (HV69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H) trimer, His,Avitag(TM) (MALS verified), Human
• Artikelnummer: ABS-SPN-C82E5-200UG
• Hersteller Artikelnummer: SPN-C82E5-200ug
• Alternativnummer: ABS-SPN-C82E5-200UG
• Hersteller: AcroBiosystems
• Wirt: Human
• Kategorie: Proteine/Peptide
• Spezies Reaktivität: Virus
• Konjugation: Biotin
• Alternative Synonym: sars-cov-2

Produktbeschreibung

Biotinylated SARSCoV2 S protein trimer, His,Avitag (SPNC82E5) is expressed from human 293 cells (HEK293). It contains AA Val 16 Pro 1213 (Accession QHD43416.1). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the Cterminus. Proline substitutions (F817P/ A892P/ A899P/ A942P/ K986P/ V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARSCoV2 S protein and abolish the furin cleavage site, respectively. The HV6970del/ Y144del/ N501Y/ A570D/ D614G/ P681H/ T716I/ S982A/ D1118H/ mutations were identified in the SARSCoV2 Alpha variant (Pango lineage: B.1.1.7, other names: 20I/501Y.V1 or VOC 202012/01). - Proteine/Peptide

Produkteigenschaften

• Molekulargewicht: 139.4 kDa
• Tag: C-10*His & C-Avi
• NCBI: 43416
• Puffer: 0.1 M Sodium citrate, pH5.5
• Reinheit: 95%
• Formulierung: Powder
• Target-Kategorie: Spike protein

Bilder

Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 1 µg/mL (100 µL/well) can bind Biotinylated SARS-CoV-2 S protein trimer, His,Avitag (Cat. No. SPN-C82E5) with a linear range of 2-78 ng/mL (QC tested).

Immobilized Biotinylated SARS-CoV-2 S protein trimer, His,Avitag (Cat. No. SPN-C82E5) at 1 µg/mL (100 µL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 µg/well) plate can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.2-3 ng/mL (Routinely tested).

Biotinylated SARS-CoV-2 S protein trimer, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.