SARS-CoV-2 Spike Antibody (AM004414)

Artikelnummer: ACM-91345
Artikelname: SARS-CoV-2 Spike Antibody (AM004414)
Artikelnummer: ACM-91345
Hersteller Artikelnummer: 91345
Alternativnummer: ACM-91345
Hersteller: Active Motif
Kategorie: Antikörper
Alternative Synonym: SARS-COV-2, CORONA, COVID-19, COV2, COVID19, SARS-COV, NCOV, COVID, COV-2

Antibody Type:





Protein A Chromatography



Molecular Weight:

141 kDa (full length S1 protein)




Western Blot Validated Enzyme-linked Immunosorbent Assay Validated

Application Notes

Note: Clone AM004414, developed by Active Motif, is also referred to as Clone 414-4.
Details on the development and characterization of this clone are available here: Wan J., et. al. Human IgG neutralizing monoclonal antibodies block SARS-CoV-2 infection. Cell Reports, 32: 107918, July 21, 2020.

Recommended Range:
WB: 0.5 - 2 µg/ml




140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, and 30% glycerol.


SARS-CoV-2 Spike Antibody (AM004414) tested by ELISA.

SARS-CoV-2 Spike Antibody (clone AM004414) tested by ELISA.
SARS-CoV-2 Spike RBD protein was coated onto microtiter plates at 0.5 µg/mL and then incubated with a dilution series of SARS-CoV-2 Spike Antibody (clone AM009105). Bound antibodies were detected with anti-human IgG conjugated to horseradish peroxidase (HRP) followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

SARS-CoV-2 Spike Antibody (AM004414) tested by Western blot.

SARS-CoV-2 Spike Antibody (AM004414) tested by Western blot.
Lysates from HEK293 cells transfected (Lane 1 and 3) or untransfected (Lane 2) was probed with 0.5ug/mL of rAb (Lane 1 & 2) or an anti-flag Ab (Lane 3).
Lane 1: transfected flag-tagged SARS-CoV-2 S1 subunit
Lane 2 untransfected
Lane 3: transfected flag-tagged SARS-CoV-2 S1 subunit


COVID-19, which is short for coronavirus disease 2019, is the official name of the respiratory disease caused by infection with the novel coronavirus SARS-CoV-2. The virus that causes COVID-19 was named SARS-CoV-2 because it is a coronavirus genetically similar to, yet distinct from, the virus that caused the severe acute respiratory syndrome (SARS) outbreak in 2003. Studying the details of how this virus replicates and causes the disease will allow scientists and physicians to more rapidly develop fast and accurate methods of detection as well as to deploy therapeutic and vaccine strategies.

This antibody was derived from COVID-19 patients who have cleared the virus. Patient serum IgG was sequenced and expressed as full-length IgG1 with human immunoglobulin heavy and light chains in mammalian 293 cells.


Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.