PsbA | D1 protein of PSII positive control/quantitation standard

Produktübersicht

• Artikelname: PsbA | D1 protein of PSII positive control/quantitation standard
• Artikelnummer: AGR-AS01-016S
• Hersteller Artikelnummer: AS01-016S
• Alternativnummer: AGR-AS01-016S
• Hersteller: Agrisera
• Kategorie: Proteine/Peptide
• Applikation: WB

Produktbeschreibung

The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination

Produkteigenschaften

• Molekulargewicht: The standard has an actual MW of 41.5 kDa, The presence of a His6 tag causes it to run ~1.7 kDa higher on the gel than the native protein, Note that in most systems, PsbA migrates with an apparent MW of between 30 and 35 kDa.
• Formulierung: Lyophilized in glycerol.
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: Standard curve: 3 loads are recommended (0.5, 2 and 4 µl).For most applications a sample load of 0.2 µg of chlorophyll will give a PsbA signal in this range.Positive control: a 2 µl load per well is optimal for most chemiluminescent detection systems.Non-
• Anwendungsbeschreibung: Concentration: after adding 95 µl of sterile milliQ water final concentration of the standard is 0.25 pmoles/µlProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.