Synthetic HDEL peptide corresponding to the C-terminus of yeast Bip
HDEL (his-asp-glu-leu) is a C-terminal tertapeptide found in yeast and plants which allows sorting of resident soluble proteins in the lumen of the endoplasmic reticulum.
Klonalität:
Monoclonal
Molekulargewicht:
78 kDa
Isotyp:
IgG2b
Reinheit:
Total IgG. Protein G purified in PBS pH 7.4.
Formulierung:
Liquid
Antibody Type:
Monoclonal Antibody
Application Verdünnung:
1: 50 - 1: 500 (IF), 1 : 100 - 1: 1000 (WB)
Anwendungsbeschreibung:
Antibody in concentration 1 µg/ml was sufficient for detection of HDEL-containing proteins in 10 µg of yeast cell lysate by colorimetric western blot. Clone 2E7.For western blot results, immunofluorescence and immunogold images please referr to Napier et al. 1992.
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