DELLA protein RGL2 is a probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Involved in the biosynthesis of abscisic acid (ABA), delays flowering and leaf production, reulates floral development, petal,
As this protein is expressed in tissue in very low levels, please load at least 50 µg/well.Recommended extraction and denaturation protocol: Arabidopsis thaliana seedlings were ground in liquid nitrogen (100 µl of 2.5 x Laemli for 80-120 mg of homogenized material) and boiled for 2 min. at 95C in 2,5x Laemmli Buffer (with 60 Mm DTT final concentration) otherwise RGA protein will degrade.
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