Anti-LHY | Late elongated hypocotyl, Rabbit, Polyclonal
Artikelnummer:
AGR-AS13-2661
Hersteller Artikelnummer:
AS13-2661
Alternativnummer:
AGR-AS13-2661
Hersteller:
Agrisera
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
WB
Spezies Reaktivität:
A. thaliana
Immunogen:
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana LHY protein sequence, UniProt: Q6R0H1-1, TAIR: AT1G01060
LHY (Late elongated hypocotyl) is a transcription factor which is involved in the circadian clock. It binds to the promoter region of APRR1/TOC1 and TCP21/CHE to repress their transcription. Alternative names: LATE ELONGATED HYPOCOTYL, LATE ELONGATED HYP
Due to biology of LHY protein it accumulates short after dawn and not in the later day, therefore proper material has to be used to obtain a signal with this antibody.Important note about protein extractionTranscription factors are best isolated by freezing nucelar extract in liquid nitrogen after adding the extraction buffer, followed by thawing directly in 100C heating block not in RT. When the samples were heated for 10 min, spinning of the extract for 5 min is needed to remove the cell debris. From the liquid extract measurement can be done and the rest, frozen in liquid Nitrogen, in aliquots. Frozen samples will not be thawed again to 100C, but on RT and loaded as soon as they are liquid. Such precautions are necessary to take to be able to detect a transcription factor using antibodies.This product can be sold containing ProClin if requested
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