HDAC6 Antibody - N-terminal region
Artikelnummer:
ASB-ARP31451_P050
- Bilder (6)
| Artikelname: | HDAC6 Antibody - N-terminal region |
| Artikelnummer: | ASB-ARP31451_P050 |
| Hersteller Artikelnummer: | ARP31451_P050 |
| Alternativnummer: | ASB-ARP31451_P050-25UL,ASB-ARP31451_P050-100UL |
| Hersteller: | Aviva |
| Wirt: | Rabbit |
| Kategorie: | Proteine/Peptide |
| Applikation: | ChIP, IHC, WB |
| Spezies Reaktivität: | Bovine, Canine, Equine, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat |
| Immunogen: | The immunogen is a synthetic peptide directed towards the N terminal region of human HDAC6 |
| Alternative Synonym: | HD6, JM21, CPBHM, PPP1R90 |
| Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription. |
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Chromatin Immunoprecipitation (ChIP) Using HDAC6 antibody - N-terminal region (ARP31451_P050) and HCT116 Cells |
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Rabbit Anti-HDAC6 Antibody Catalog Number: ARP31451_P050 Formalin Fixed Paraffin Embedded Tissue: Human Adult heart Observed Staining: Cytoplasmic,Nuclear Primary Antibody Concentration: 1:600 Secondary Antibody: Donkey anti-Rabbit-Cy2/3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 a€'' 2.0 sec Protocol located in Reviews and Data. |
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Rabbit Anti-HDAC6 Antibody Catalog Number: ARP31451_P050 Formalin Fixed Paraffin Embedded Tissue: Human Kidney Tissue Observed Staining: Cytoplasm Primary Antibody Concentration: 1:100 Other Working Concentrations: N/A Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec |
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WB Suggested Anti-HDAC6 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Positive Control: 293T cell lysateThere is BioGPS gene expression data showing that HDAC6 is expressed in HEK293T |
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Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown. |
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HDAC6 antibody - N-terminal region (ARP31451_P050) in Human 293T using Western Blot |
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