SP7 Antibody - N-terminal region

Produktübersicht

• Artikelname: SP7 Antibody - N-terminal region
• Artikelnummer: ASB-ARP32231_P050
• Hersteller Artikelnummer: ARP32231_P050
• Alternativnummer: ASB-ARP32231_P050-25UL,ASB-ARP32231_P050-100UL
• Hersteller: Aviva
• Wirt: Rabbit
• Kategorie: Proteine/Peptide
• Applikation: IHC, WB
• Spezies Reaktivität: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SP7
• Alternative Synonym: OSX, OI11, OI12, osterix

Produktbeschreibung

SP7 is a C2H2-type zinc finger transcription factor of the SP gene family and a putative master regulator of bone cell differentiation.

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.5 mg/ml
• Molekulargewicht: 45 kDa
• NCBI: 121340
• UniProt: Q8TDD2
• Formulierung: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Bilder

25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. An isoform containing the peptide sequence is present at 43 kDa.

Host: Rabbit
Target Name: SP7
Sample Tissue: Human Fetal Lung
Antibody Dilution: 1.0ug/ml

Immunohistochemistry with Testis tissue at an antibody concentration of 5ug/ml using anti-SP7 antibody (ARP32231_P050)

WB Suggested Anti-SP7 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Human kidney

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

SP7 antibody - N-terminal region (ARP32231_P050) in Human Fetal Lung using Western Blot