UBE2N Antibody - middle region

Produktübersicht

• Artikelname: UBE2N Antibody - middle region
• Artikelnummer: ASB-ARP33126_T100
• Hersteller Artikelnummer: ARP33126_T100
• Alternativnummer: ASB-ARP33126_T100-25UL,ASB-ARP33126_T100-100UL
• Hersteller: Aviva
• Wirt: Rabbit
• Kategorie: Proteine/Peptide
• Applikation: IHC, WB
• Spezies Reaktivität: Bovine, Canine, Equine, Goat, Guinea pig, Human, Mouse, Rabbit, Rat, Sheep, Yeast, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human UBE2N
• Alternative Synonym: UBC13, UbcH13, HEL-S-71, UbcH-ben, UBCHBEN, UBC13

Produktbeschreibung

UBE2N encodes a member of the E2 ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/ml
• Molekulargewicht: 17kDa
• NCBI: 7334
• UniProt: P61088
• Formulierung: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Bilder

Sample Type:
Mouse brain stem cellsPrimary Antibody Dilution:
1:500Secondary Antibody:
Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution:
1:1000Color/Signal Descriptions:
UBE2N: Red DAPI:BlueGene Name:
UBE2NSubmitted by:
Dr. Yuzhi Chen, University of Arkansas for Medical Science

WB Suggested Anti-UBE2N Antibody Titration: 2.5ug/ml
Positive Control: Jurkat cell lysateUBE2N is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells

Sample Type: 1. Human NT-2 cells (60ug)
2. mouse brain extracts (80ug)
Primary Antibody Dilution: ,2ug/ml
Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience
Secondary Antibody Dilution: ,1: 20,000
Image Submitted by: Yuzhi Chen
University of Arkansas for Medical Science ,

Lanes:
1: 40ng HIS-UBE2D1 protein
2: 40ng HIS-UBE2D2 protein
3: 40ng HIS-UBE2D3 protein
4: 40ng HIS-UBE2D4 protein
5: 40ng HIS-UBE2E1 protein
6: 40ng HIS-UBE2E2 protein
7: 40ng HIS-UBE2E3 protein
8: 40ng HIS-UBE2K protein
9: 40ng HIS-UBE2L3 protein
10: 40ng HIS-UBE2N protein
11: 40ng HIS-UBE2V1 protein
12: 40ng HIS-UBE2V2 protein.
Primary Antibody Dilution:
1:500
Secondary Antibody:
Anti-rabbit-HRP
Secondary Antibody Dilution:
1:50,000
Gene Name:
UBE2N
Submitted by:
Dr Chris Boutell. MRC-UoG Centre for Virus Research (CVR), UK.

UBE2N antibody - middle region (ARP33126_T100) in Human Jurkat using Western Blot