SP3 Antibody - middle region

Artikelnummer: ASB-ARP33336_P050
Artikelname: SP3 Antibody - middle region
Artikelnummer: ASB-ARP33336_P050
Hersteller Artikelnummer: ARP33336_P050
Alternativnummer: ASB-ARP33336_P050-25UL,ASB-ARP33336_P050-100UL
Hersteller: Aviva
Wirt: Rabbit
Kategorie: Proteine/Peptide
Applikation: IHC, WB
Spezies Reaktivität: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat
Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SP3
Alternative Synonym: SPR2
SP3 is a transcriptional factor that can act as an activator or repressor, probably in a isoform-specific manner. SP3 binds to GT and GC boxes promoters elements.This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted.
Klonalität: Polyclonal
Konzentration: 0.5 mg/ml
Molekulargewicht: 82 kDa
NCBI: 6670
UniProt: Q02447
Formulierung: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Immunohistochemistry with Human Prostate lysate tissue at an antibody concentration of 5.0ug/ml using anti-SP3 antibody (ARP33336_P050)
WB Suggested Anti-SP3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Human Muscle
Sample Type: Human nuclear cell extracts (30ug)
Primary Dilution: 1:1000
Secondary Antibody: anti-Rabbit HRP
Secondary Dilution: 1:20000
Image Submitted By: AKatarina Luciakova
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SP3 is supported by BioGPS gene expression data to be expressed in U2OS
Host: Mouse
Target Name: SP3
Sample Tissue: Mouse Skeletal Muscle
Antibody Dilution: 1ug/ml
25 ug of the indicated Mouse whole tissue extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. Multiple isoforms (~6) of mouse Sp3 are known and several can be identified with this antibody.
SP3 antibody - middle region (ARP33336_P050) in Human Muscle using Western Blot