TBX1 Antibody - C-terminal region

Produktübersicht

• Artikelname: TBX1 Antibody - C-terminal region
• Artikelnummer: ASB-ARP33399_T100
• Hersteller Artikelnummer: ARP33399_T100
• Alternativnummer: ASB-ARP33399_T100-25UL,ASB-ARP33399_T100-100UL
• Hersteller: Aviva
• Wirt: Rabbit
• Kategorie: Proteine/Peptide
• Applikation: IHC, WB
• Spezies Reaktivität: Canine, Guinea pig, Human, Mouse, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human TBX1
• Alternative Synonym: DGS, TGA, VCF, CAFS, CTHM, DGCR, DORV, VCFS, TBX1C, CATCH22

Produktbeschreibung

TBX1 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. TBX1 product shares 98% amino acid sequence identity with the mouse ortholog. DiGeorge syndrome (DGS)/velocardiofacial syndrome (VCFS), a common congenital disorder characterized by neural-crest-related developmental defects, has been associated with deletions of chromosome 22q11.2, where TBX1 has been mapped. Studies using mouse models of DiGeorge syndrome suggest a major role for this gene in the molecular etiology of DGS/VCFS. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/ml
• Molekulargewicht: 43 kDa
• NCBI: 6899
• UniProt: O43435-3
• Formulierung: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Bilder

25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 5 ug/mL of the antibody was used in this experiment.

Human kidney

WB Suggested Anti-TBX1 Antibody Titration: 5.0ug/ml
Positive Control: Jurkat cell lysate

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

TBX1 antibody - C-terminal region (ARP33399_T100) in Human Jurkat using Western Blot