Gap junction protein, alpha 1 is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5.
Species+tissue/cell type: Total rat cardiac lysate 1: 8 ug total cardiac lysate 2: 15 ug total cardiac lysate 3: 30 ug total cardiac lysate 4: 50 ug total cardiac lysatePrimary antibody dilution: 0.25 ug/ml
Lanes: Lane1: 8 ug total cardiac lysate Lane2: 15 ug total cardiac lysate Lane3: 30 ug total cardiac lysate Lane4: 50 ug total cardiac lysate Primary Antibody Dilution: 0.25 ug/ml Secondary Antibody:
Secondary Antibody Dilution:
Gene Name: GJA1 Submitted by: Anonymous
WB Suggested Anti-GJA1 Antibody Positive Control: Lane1: 60ug rat stiatum Primary Antibody Dilution : 1:1000 Secondary Antibody : Goat anti-rabbit-IRDye800 Secondry Antibody Dilution : 1:10,000 Submitted by: Ruben van Vugt, The Nijmegen Centre for Molecular Life Sciences (NCMLS)
GJA1 antibody - N-terminal region (ARP36598_P050) in Human Placenta using Western Blot
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