STAT3 Antibody - N-terminal region
Artikelnummer:
ASB-ARP38253_P050
- Bilder (9)
| Artikelname: | STAT3 Antibody - N-terminal region |
| Artikelnummer: | ASB-ARP38253_P050 |
| Hersteller Artikelnummer: | ARP38253_P050 |
| Alternativnummer: | ASB-ARP38253_P050-25UL,ASB-ARP38253_P050-100UL |
| Hersteller: | Aviva |
| Wirt: | Rabbit |
| Kategorie: | Proteine/Peptide |
| Applikation: | IF, IHC, WB |
| Spezies Reaktivität: | Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish |
| Immunogen: | The immunogen is a synthetic peptide directed towards the N terminal region of human STAT3 |
| Alternative Synonym: | APRF, HIES, ADMIO, ADMIO1 |
| STAT3 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. It mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein.The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. |
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25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. The immunizing peptideIs present in multiple isoforms from 83-88 kDa, as well as contained within a 49 kDa smaller i |
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Immunohistochemistry with Human kidney lysate tissue |
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Immunohistochemistry with Human Uterus lysate tissue |
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Immunohistochemistry with human prostate tissue tissue |
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Immunofluorescence --Sample Type: Macrophages Dilution: 1:1000 |
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WB Suggested Anti-STAT3 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:1562500 Positive Control: Human Liver |
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Host: Rabbit Target: STAT3 Positive control (+): A549 (N03) Negative control (-): HepG2 (HG) Antibody concentration: 1ug/ml |
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Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 0.5, 5, and 50 ug/mL. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for either two or three concentrations were averaged and results and standard deviation are shown. |
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STAT3 antibody - N-terminal region (ARP38253_P050) in Human Liver using Western Blot |
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