TEAD4 Antibody - C-terminal region

Produktübersicht

• Artikelname: TEAD4 Antibody - C-terminal region
• Artikelnummer: ASB-ARP38276_P050
• Hersteller Artikelnummer: ARP38276_P050
• Alternativnummer: ASB-ARP38276_P050-25UL,ASB-ARP38276_P050-100UL
• Hersteller: Aviva
• Wirt: Rabbit
• Kategorie: Proteine/Peptide
• Applikation: IHC, WB
• Spezies Reaktivität: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human TEAD4
• Alternative Synonym: TEF3, RTEF1, TEF-3, EFTR-2, TEFR-1, TCF13L1, hRTEF-1B

Produktbeschreibung

TEAD4 is a member of the transcriptional enhancer factor (TEF) family. The family members contain the TEA/ATTS DNA-binding domain. TEAD4 is preferentially expressed in skeletal muscle, and binds to the M-CAT regulatory element which directs muscle-specific gene expression. TEAD4 is encoded through the use of a non-AUG (TTG) translation initiation codon. This gene encodes a member of the transcriptional enhancer factor (TEF) family. The family members contain the TEA/ATTS DNA-binding domain. This member is preferentially expressed in skeletal muscle, and binds to the M-CAT regulatory element which directs muscle-specific gene expression. The protein is encoded through the use of a non-AUG (TTG) translation initiation codon. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.5 mg/ml
• Molekulargewicht: 48kDa
• NCBI: 7004
• UniProt: Q96BK2
• Formulierung: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Bilder

25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. The canonical 48 kDa isoform contains this peptide sequence, as do 44 and 34 kDa isoforms.

Host: Rabbit
Target Name: TEAD4
Sample Tissue: Human Ovary Tumor
Antibody Dilution: 1ug/ml

Immunohistochemistry with Human Pancrease lysate tissue at an antibody concentration of 5.0ug/ml using anti-TEAD4 antibody (ARP38276_P050)

WB Suggested Anti-TEAD4 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: 293T cell lysateTEAD4 is supported by BioGPS gene expression data to be expressed in HEK293T

Host: Rabbit
Target Name: TEAD4
Sample Type: 293T
Lane A: Primary Antibody
Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration: 1ug/ml
Peptide Concentration: 5ug/ml
Lysate Quantity: 25ug/lane/lane
Gel Concentration: 0.12

Host: Rabbit
Target: TEAD4
Positive control (+): 293T Cell Lysate (2T)
Negative control (-): Human Stomach Tumor (T-ST)
Antibody concentration: 3ug/ml

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

TEAD4 antibody - C-terminal region (ARP38276_P050) in Human Ovary Tumor using Western Blot