SLC10A1 Antibody - middle region

Produktübersicht

• Artikelname: SLC10A1 Antibody - middle region
• Artikelnummer: ASB-ARP42097_P050
• Hersteller Artikelnummer: ARP42097_P050
• Alternativnummer: ASB-ARP42097_P050-25UL,ASB-ARP42097_P050-100UL
• Hersteller: Aviva
• Wirt: Rabbit
• Kategorie: Proteine/Peptide
• Applikation: WB
• Spezies Reaktivität: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SLC10A1
• Alternative Synonym: NTCP, FHCA2

Produktbeschreibung

Sodium/bile acid cotransporters are integral membrane glycoproteins that participate in the enterohepatic circulation of bile acids. Two homologous transporters are involved in the reabsorption of bile acids, one absorbing from the intestinal lumen, the b

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.5 mg/ml
• Molekulargewicht: 38 kDa
• NCBI: 6554
• UniProt: Q14973
• Formulierung: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Bilder

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. SLC10A1 is modified by N-linked glycosylation.

WB Suggested Anti-SLC10A1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: HT1080 cell lysate

Host: Rabbit
Target Name: SLC10A1
Sample Type: HT1080
Lane A: Primary Antibody
Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration: 1ug/ml
Peptide Concentration: 5ug/ml
Lysate Quantity: 25ug/lane/lane
Gel Concentration: 0.12

Host: Rabbit
Target Name: SLC10A1
Sample Tissue: Human MDA-MB-435s
Antibody Dilution: 1.0ug/ml

Host: Rabbit
Target: SLC10A1
Positive control (+): Human liver (LI)
Negative control (-): 293T (2T)
Antibody concentration: 1ug/ml

Host: Rabbit
Target Name: SLC10A1
Sample Tissue: Human Jurkat Whole Cell
Antibody Dilution: 1ug/ml

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

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