IDH3A Antibody - N-terminal region
Artikelnummer:
ASB-ARP42237_T100
- Bilder (7)
| Artikelname: | IDH3A Antibody - N-terminal region |
| Artikelnummer: | ASB-ARP42237_T100 |
| Hersteller Artikelnummer: | ARP42237_T100 |
| Alternativnummer: | ASB-ARP42237_T100-25UL,ASB-ARP42237_T100-100UL |
| Hersteller: | Aviva |
| Wirt: | Rabbit |
| Kategorie: | Proteine/Peptide |
| Applikation: | IHC, IP, WB |
| Spezies Reaktivität: | Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish |
| Immunogen: | The immunogen is a synthetic peptide directed towards the N terminal region of human IDH3A |
| Alternative Synonym: | RP90 |
| Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. IDH3A is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. |
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Rabbit Anti-IDH3A Antibody Catalog Number: ARP42237 Paraffin Embedded Tissue: Human Muscle Cellular Data: Skeletal muscle cells Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X |
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WB Suggested Anti-IDH3A Antibody Titration: 1.25ug/ml Positive Control: Jurkat cell lysateIDH3A is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells |
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Host: Rabbit Target Name: IDH3A Sample Type: Jurkat Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 2.5ug/mL Peptide Concentration: 2.0ug/mL Lysate Quantity: 25ug/lane Gel Concentration: 12% |
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Host: Rabbit Target: IDH3A Positive control (+): 293T (2T) Negative control (-): Liver tumor (T-LI) Antibody concentration: 1ug/ml |
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IDH3A was immunoprecipitated from 2 mg HEK293 Whole Cell Lysate with ARP42237_T100 with 1:200 dilution. Western blot was performed using ARP42237_T100 at 1/1000 dilution. Lane 1: Control IP in HEK293 Whole Cell Lysate. Lane 2: IDH3A IP with ARP42237_T100 in HEK293 Whole Cell Lysate. Lane 3: Input of HEK293 Whole Cell Lysate. |
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Host: Rabbit Target Name: IDH3A Sample Tissue: Human HepG2 Antibody Dilution: 1.0ug/ml |
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IDH3A Antibody - N-terminal region (ARP42237_T100) in 293T, Liver tumor using Western Blot |
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