PLOD2 Antibody - middle region

Produktübersicht

• Artikelname: PLOD2 Antibody - middle region
• Artikelnummer: ASB-ARP42731_P050
• Hersteller Artikelnummer: ARP42731_P050
• Alternativnummer: ASB-ARP42731_P050-25UL,ASB-ARP42731_P050-100UL
• Hersteller: Aviva
• Wirt: Rabbit
• Kategorie: Proteine/Peptide
• Applikation: IHC, WB
• Spezies Reaktivität: Bovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PLOD2
• Alternative Synonym: LH2, TLH, BRKS2

Produktbeschreibung

PLOD2 forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. Mutations in the coding region of this gene are associated with Bruck syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.5 mg/ml
• Molekulargewicht: 85 kDa
• NCBI: 5352
• UniProt: O00469-2
• Formulierung: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Bilder

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 4 ug/mL of the antibody was used in this experiment.

Rabbit Anti-PLOD2 Antibody
Catalog Number: ARP42731_P050
Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue
Observed Staining: Cytoplasmic
Primary Antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

WB Suggested Anti-PLOD2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: DU145 cell lysatePLOD2 is strongly supported by BioGPS gene expression data to be expressed in Human DU145 cells

Host: Rabbit
Target: PLOD2
Positive control (+): 293T (2T)
Negative control (-): THP-1 (N30)
Antibody concentration: 4ug/ml

PLOD2 antibody - middle region (ARP42731_P050) in Human DU145 using Western Blot