PEMT Antibody - C-terminal region

Produktübersicht

• Artikelname: PEMT Antibody - C-terminal region
• Artikelnummer: ASB-ARP43558_P050
• Hersteller Artikelnummer: ARP43558_P050
• Alternativnummer: ASB-ARP43558_P050-25UL,ASB-ARP43558_P050-100UL
• Hersteller: Aviva
• Wirt: Rabbit
• Kategorie: Proteine/Peptide
• Applikation: WB
• Spezies Reaktivität: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat, Yeast, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human PEMT
• Alternative Synonym: PLMT, PNMT, PEAMT, PEMPT, PEMT2

Produktbeschreibung

PEMT is an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. The protein localizes to the endoplasmic reticulum and mitochondria-associated membranes. The gene encodes PEMT protein is within the Smith-Magenis syndrome region on chromosome 17. This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. The protein localizes to the endoplasmic reticulum and mitochondria-associated membranes. The gene is within the Smith-Magenis syndrome region on chromosome 17. Alternate splicing of this gene results in three transcript variants encoding two different isoforms.

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.5 mg/ml
• Molekulargewicht: 22 kDa
• NCBI: 10400
• UniProt: Q9BW86
• Formulierung: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Bilder

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. The peptide is present in isoforms of 84, 83, 82, 71, and 42 kDa.

WB Suggested Anti-PEMT Antibody Titration: 0.2-1 ug/ml
Positive Control: 721_B cell lysate

Lanes:
Lane1: 20 ug rat liver lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-rabbit HRP
Secondary Antibody Dilution:
1:15000
Gene Name:
PEMT
Submitted by:
Anonymous

Lanes:
Lane1: 20 ug rat liver lysate
Primary Antibody Dilution:
1:2000
Secondary Antibody:
Anti-rabbit HRP
Secondary Antibody Dilution:
1:15000
Gene Name:
PEMT
Submitted by:
Anonymous

Host: Rabbit
Target Name: PEMT
Sample Tissue: Human Hela Whole Cell
Antibody Dilution: 2ug/ml

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

PEMT antibody - C-terminal region (ARP43558_P050) in Human 721_B using Western Blot