CYP46A1 Antibody - C-terminal region

Produktübersicht

• Artikelname: CYP46A1 Antibody - C-terminal region
• Artikelnummer: ASB-ARP43670_P050
• Hersteller Artikelnummer: ARP43670_P050
• Alternativnummer: ASB-ARP43670_P050-25UL,ASB-ARP43670_P050-100UL
• Hersteller: Aviva
• Wirt: Rabbit
• Kategorie: Proteine/Peptide
• Applikation: WB
• Spezies Reaktivität: Bovine, Canine, Equine, Goat, Guinea pig, Human, Mouse, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human CYP46A1
• Alternative Synonym: CP46, CYP46

Produktbeschreibung

CYP46A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids.This protein localizes to the endoplasmic reticulum and hydroxylates medium-chain fatty acids such as laurate and myristate.This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein is expressed in the brain, where it converts cholesterol to 24S-hydroxycholesterol. While cholesterol cannot pass the blood-brain barrier, 24S-hydroxycholesterol can be secreted in the brain into the circulation to be returned to the liver for catabolism. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.5 mg/ml
• Molekulargewicht: 57 kDa
• NCBI: 10858
• UniProt: Q9Y6A2
• Formulierung: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Bilder

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 0.5 ug/mL of the antibody was used in this experiment.

WB Suggested Anti-CYP46A1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: HepG2 cell lysate

Host: Rabbit
Target Name: SERPINA3
Sample Type: Human Adult Placenta
Antibody Dilution: 1.0ug/ml

Host: Rabbit
Target Name: CYP46A1
Sample Tissue: Human PANC1
Antibody Dilution: 1.0ug/ml

Host: Rabbit
Target: CYP46A1
Positive control (+): Human heart (HE)
Negative control (-): Human Placenta (PL)
Antibody concentration: 1ug/ml

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

CYP46A1 antibody - C-terminal region (ARP43670_P050) in Human PANC1 using Western Blot