SLC24A6 Antibody - middle region

Artikelnummer: ASB-ARP44042_P050
Artikelname: SLC24A6 Antibody - middle region
Artikelnummer: ASB-ARP44042_P050
Hersteller Artikelnummer: ARP44042_P050
Alternativnummer: ASB-ARP44042_P050-25UL,ASB-ARP44042_P050-100UL
Hersteller: Aviva
Wirt: Rabbit
Kategorie: Proteine/Peptide
Applikation: WB
Spezies Reaktivität: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat
Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SLC24A6
Alternative Synonym: NCLX, NCKX6, SLC24A6
SLC24A6 belongs to a family of potassium-dependent sodium/calcium exchangers that maintain cellular calcium homeostasis through the electrogenic countertransport of 4 sodium ions for 1 calcium ion and 1 potassium ion.SLC24A6 belongs to a family of potassium-dependent sodium/calcium exchangers that maintain cellular calcium homeostasis through the electrogenic countertransport of 4 sodium ions for 1 calcium ion and 1 potassium ion (Cai and Lytton, 2004 [PubMed 14625281]).[supplied by OMIM].
Klonalität: Polyclonal
Konzentration: 0.5 mg/ml
Molekulargewicht: 64 kDa
NCBI: 80024
UniProt: Q6J4K2
Formulierung: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
WB Suggested Anti-SLC24A6 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: 721_B cell lysate
Host: Rabbit
Target: SLC8B1
Positive control (+): Human Placenta (PL)
Negative control (-): 293T (2T)
Antibody concentration: 1ug/ml
25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 5 ug/mL of the antibody was used in this experiment. The canonical isoform of 64 kDa is present as well as a second isoform around 58 kDa.
Host: Rabbit
Target Name: SLC24A6
Sample Tissue: Human 786-0 Whole Cell
Antibody Dilution: 5ug/ml
Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.
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