SHH Antibody - N-terminal region

Artikelname:	SHH Antibody - N-terminal region
Artikelnummer:	ASB-ARP44235_P050
Hersteller Artikelnummer:	ARP44235_P050
Alternativnummer:	ASB-ARP44235_P050-25UL,ASB-ARP44235_P050-100UL
Hersteller:	Aviva
Wirt:	Rabbit
Kategorie:	Proteine/Peptide
Applikation:	IHC, WB
Spezies Reaktivität:	Bovine, Canine, Equine, Gallus, Goat, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish
Immunogen:	The immunogen is a synthetic peptide directed towards the N terminal region of human SHH
Alternative Synonym:	TPT, HHG1, HLP3, HPE3, SMMCI, ShhNC, TPTPS, MCOPCB5

SHH is a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE). It is also thought that mutations in its gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.This gene, which is expressed only during embryogenesis, encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved, the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. In addition, it is thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.

Klonalität:	Polyclonal
Konzentration:	0.5 mg/ml
Molekulargewicht:	28 kDa
NCBI:	6469
UniProt:	Q15465
Formulierung:	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

	Chicken embryos Primary antibody: 1:1000 (ARP44235_p050) anti -shhSecondary Antibody: 1:500 (ASP00001) goat anti-rabbit HRP conjugated
	Sample Type: Human glioma cellsPrimary Antibody Dilution: 1:200Secondary Antibody: Anti-rabbit-GFPSecondary Antibody Dilution: 1:500Color/Signal Descriptions: 1. SHH: Green 2. DAPI: Blue 3. MergeGene Name: SHHSubmitted by: ELIAS EL HABR, PhD, Post-Doctoral fellow, INSERM U894 Glial Plasticity Team, Psychiatry& Neurosciences Center, St Anne Hospital, 2 Ter, Rue dAlesia, 75014 Paris
	WB Suggested Anti-SHH Antibody Titration: 0.2-1 ug/ml Positive Control: HepG2 cell lysate
	Host: Rabbit Target Name: SHH Sample Tissue: Human Stomach Tumor Antibody Dilution: 1.0ug/ml
	Host: Mouse Target Name: SHH Sample Tissue: Mouse Brain Antibody Dilution: 1ug/ml
	Rabbit Anti-SHH Antibody Catalog Number: ARP44235_P050 Formalin Fixed Paraffin Embedded Tissue: Human Urinary Bladder Tissue Observed Staining: Plasma membrane, Cytoplasm Primary Antibody Concentration: 1:100 Other Working Concentrations: N/A Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec
	Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.
	SHH antibody - N-terminal region (ARP44235_P050) in Human Stomach Tumor using Western Blot