CY24B Antibody - C-terminal region

Produktübersicht

• Artikelname: CY24B Antibody - C-terminal region
• Artikelnummer: ASB-ARP44276_P050
• Hersteller Artikelnummer: ARP44276_P050
• Alternativnummer: ASB-ARP44276_P050-25UL,ASB-ARP44276_P050-100UL
• Hersteller: Aviva
• Wirt: Rabbit
• Kategorie: Proteine/Peptide
• Applikation: WB
• Spezies Reaktivität: Human
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human CY24B
• Alternative Synonym: CGD, NOX2, IMD34, AMCBX2, GP91-1, GP91PHOX, p91-PHOX, GP91-PHOX

Produktbeschreibung

Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase, neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cells respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole.

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.5 mg/ml
• Molekulargewicht: 62 kDa
• NCBI: 1536
• UniProt: P04839
• Formulierung: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Bilder

IHC Information: Lung, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)

Host: Rabbit
Target Name: CY24B
Sample Tissue: HepG2 Whole Cell lysates
Antibody Dilution: 1.0ug/ml

Host: Rabbit
Target Name: CYBB
Sample Tissue: Human THP-1 Whole Cell
Antibody Dilution: 1ug/ml

Host: Rabbit
Target Name: CYBB
Sample Tissue: Human Jurkat Whole Cell
Antibody Dilution: 1ug/ml

Host: Rabbit
Target Name: CYBB
Sample Tissue: Human PANC1 Whole Cell
Antibody Dilution: 1ug/ml

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 4 ug/mL of the antibody was used in this experiment.

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

CY24B Antibody - C-terminal region (ARP44276_P050) in Human HepG2 Whole Cell using Western Blot