POR Antibody - middle region
Artikelnummer:
ASB-ARP44362_P050
- Bilder (7)
| Artikelname: | POR Antibody - middle region |
| Artikelnummer: | ASB-ARP44362_P050 |
| Hersteller Artikelnummer: | ARP44362_P050 |
| Alternativnummer: | ASB-ARP44362_P050-25UL,ASB-ARP44362_P050-100UL |
| Hersteller: | Aviva |
| Wirt: | Rabbit |
| Kategorie: | Proteine/Peptide |
| Applikation: | IHC, WB |
| Spezies Reaktivität: | Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish |
| Immunogen: | The immunogen is a synthetic peptide directed towards the middle region of human POR |
| Alternative Synonym: | CPR, CYPOR, P450R |
| POR is an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this POR gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome.This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
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ARP44362_P050 |
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WB Suggested Anti-POR Antibody Titration: 0.25ug/ml Positive Control: 293T cells lysate |
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25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. |
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Host: Rabbit Target Name: POR Sample Tissue: Human HCT116 Whole Cell Antibody Dilution: 3ug/ml |
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Host: Rabbit Target Name: POR Sample Tissue: Human MCF7 Whole Cell Antibody Dilution: 3ug/ml |
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Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown. |
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POR antibody - middle region (ARP44362_P050) in Human 293T using Western Blot |
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